Identification that KfiA, a Protein Essential for the Biosynthesis of the Escherichia coli K5 Capsular Polysaccharide, is an alpha -UDP-GlcNAc Glycosyltransferase: The Formation of a Membrane Associated K5 Biosynthetic Complex Requires KfiA, KfiB and KfiC
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چکیده
منابع مشابه
Expression of E.coli capsular polysaccharide requires the KfiB protein:A Structural based analysis
Abstract Background and objectives: important virulence factor for many invasive bacterial pathogens of humans. Escherichia coli offer a model system to study the mechanisms by which capsular polysaccharides are synthesized and exported onto the cell surface of bacteria. Biosynthesis of the E consists of the repeat structure -4) GlcA- (1, 4)-GlcNAc- (1-, requires the KfiA,...
متن کاملBiosynthesis of the Escherichia coli K5 capsular polysaccharide.
299-304 47 Payne, G., Virji, M. and Saunden, J. R ( I 996) in Abstracts of the Tenth International Pathogenic Neisseria Conference (Zollinger, W.D., Frasch, C.E. and Deal, C.D., eds.), pp. 393-394, National Institutes of Health, Bethesda, MD 48 Parge, H. E.. Forest, K T.. Hickey, M. 1.. Christensen, D. A,. Getzoff, E. D. and Tainer, J. A. (I 995) Nature (London) 378, 32-38 49 Forest, K T. and T...
متن کاملOverexpression of UDP-glucose dehydrogenase in Escherichia coli results in decreased biosynthesis of K5 polysaccharide.
The Escherichia coli K5 capsular polysaccharide (glycosaminoglycan) chains are composed of the repeated disaccharide structure: -GlcAbeta1,4-GlcNAcalpha1,4-(where GlcA is glucuronic acid and GlcNAc is N-acetyl-D-glucosamine). The GlcA, present in most glycosaminoglycans, is donated from UDP-GlcA, which, in turn, is generated from UDP-glucose by the enzyme UDP-glucose dehydrogenase (UDPGDH). The...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.m004426200